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Image Search Results
Journal: Nature Aging
Article Title: Conserved epigenetic hallmarks of T cell aging during immunity and malignancy
doi: 10.1038/s43587-024-00649-5
Figure Lengend Snippet: a , Schema for generating ML memory CD8 + T cells. Naive mice were infected with VSV generating antigen-specific memory CD8 + T cells. Heterologous VSV infection and boosting of the memory T cells was performed after 30 versus 60 days post previous infection. After three infections, the antigen-specific memory CD8 + T cells were transferred into congenically distinct naive mice, and the boosting strategy was repeated over a time frame that yielded memory T cells with age ranging between 0.5 and ~4 mouse lifetime (LT) equivalents. For young memory, n = 6; 0.5× LT, n = 3; 2–3× LT, n = 5; and 4× LT, n = 3. b , Representative FACS analysis of cell trace violet (CTV) dilution from 3° (CD45.2) and 51° (CD45.1) memory T cells transferred into recipient CD45.1/2 mice. CTV label was measured 10 days after VSV-Indiana challenge of the chimeric mice. c , PCA plot of 3,000 most variable CpG sites comparing ML and ‘young’ memory T cells. Plot showing principal components 1 and 2 (PC1 and PC2). d , Violin plots showing average genome-wide CpG methylation for naive, young memory and ML CD8 + T cells. The red triangles represent the median values. e , Heatmap showing top ML gain of methylation DMRs. f , GSEA using cell cycle regulators and gain versus loss of methylation DMRs for 4× LT memory CD8 + T cells relative to young memory CD8 + T cells. The P value is based on the weighted Kolmogorov–Smirnov statistic with no adjustment. g , Genomic and CpG island annotation of top loss and gain of methylation ML DMRs. h , Summary graph showing mean methylation across top ML gain of methylation DMRs relative to boosting and age of memory T cells. Different lengths of resting period also result in significant changes of mean methylation levels. The P value is based on the two-sided Student’s t -test.
Article Snippet: The purified CD8 + T cells were then stained with anti-mouse CD8a (53-6.7) from BD Biosciences, anti-mouse CD45.1 (A20),
Techniques: Infection, Genome Wide, CpG Methylation Assay, Methylation